Ribonucleoside-Vanadyl Complex from New England Biolabs

Product Specs
ItemRibonucleoside-Vanadyl Complex
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS1402S
Quantity10 ml
ApplicationsFunctional Genomics
Concentration200 mM
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Profiling stress-triggered RNA condensation with photocatalytic proximity labelingNature CommunicationsNovember 15, 2023Ziqi Ren, Wei Tang, Luxin Peng, Peng Zoufor 20 min in PBSM containing 0.1% Triton X-100 and 2 mM vanadyl ribonucleoside complex (VRC, NEB, S1402S). After washed by PBSM, cells were then incubated at room temperature for 15 min by prehybridization-30

(2) A mitochondrial pentatricopeptide repeat protein enhances cold tolerance by modulating mitochondrial superoxide in riceNature CommunicationsOctober 25, 2023Xiaofeng Zu, Lilan Luo, Zhen Wang, Jie Gong, Chao Yang, Yong Wang, Chunhui Xu, Xinhua Qiao, Xian Deng, Xianwei Song, et al.(GE). Next, 1 mL of extraction buffer (1×PBS, 1% CA630, 1 mM PMSF, 1 mM DTT, RNase inhibitor (NEB, S1402S) with 1× protease inhibitor cocktail (Sigma-Aldrich, P9599) was added to each sample and incubated for

(3) Spatial atlas of the mouse central nervous system at molecular resolutionNatureOctober 19, 2023Hailing Shi, Yichun He, Yiming Zhou, Jiahao Huang, Kamal Maher, Brandon Wang, Zefang Tang, Shuchen Luo, Peng Tan, Morgan Wu, et al.formamide (Calbiochem, 344206), 1% Triton X-100, 20 mM ribonucleoside vanadyl complex (New England Biolabs, S1402S), 0.1 mg ml−1 yeast tRNA, 0.1 U µl−1 SUPERaseIn, and SNAIL probes) at 40 °C for 24–36 h with gentle

(4) Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCRBIO-PROTOCOLSeptember 5, 2023Ke-En Tan, Wei Lun Ng, Chee-Kwee Ea, Yat-Yuen LimD0632)NP-40 (Sigma-Aldrich, catalog number: 9016-45-9)Ribonucleoside vanadyl complexes (NEB, catalog number: S1402S)Sodium acetate (Fisher Scientific, catalog number: BP333-500)Molecular grade ethanol (Merck, catalog

(5) Spatial transcriptomics for profiling the tropism of viral vectors in tissuesNature BiotechnologySeptember 1, 2023Min J. Jang, Gerard M. Coughlin, Cameron R. Jackson, Xinhong Chen, Miguel R. Chuapoco, Julia L. Vendemiatti, Alexander Z. Wang, Viviana Gradinarucitrate (SSC, AM9763, Invitrogen), formamide (AM9342, Invitrogen), Ribonucleoside Vanadyl Complex (RVC, S1402S, New England Biolabs (NEB)), salmon sperm DNA (15632011, Invitrogen), T4 ligase (EL0011, Thermo Fisher Scientific

(6) lncRNA read-through regulates the BX-C insulator Fub-1eLifeAugust 10, 2023Airat Ibragimov, Xin Yang Bing, Yulii V Shidlovskii, Michael Levine, Pavel Georgiev, Paul SchedlDNA,Thermo Fisher Scientific,AM9680, "Chemical compound, drug",Ribonucleoside Vanadyl Complex,NEB,S1402S, "Chemical compound, drug",Nuclease-free BSA,Sigma,126609, "Chemical compound, drug",Triethylammonium

(7) The minimal intrinsic stochasticity of constitutively expressed eukaryotic genes is sub-PoissonianSci AdvAugust 9, 2023Douglas E. Weidemann, James Holehouse, Abhyudai Singh, Ramon Grima, Silke Haufspheroplast buffer [1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex (NEB, S1402S), and 20 μM beta-mercaptoethanol], and 1.2 to 5 μl of 100T zymolyase (10 mg/ml; US Biological, Z1005)

(8) Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stallingNucleic acids researchJuly 7, 2023Kejiou NS, Ilan L, Aigner S, Luo E, Tonn T, Ozadam H, Lee M, Cole GB, Rabano I, Rajakulendran N, et al.followed by a 1 h digestion at 4°C. Immediately following digestion, ribonucleoside vanadyl complex (NEB S1402S) (20 mM) was added to inhibit further digestion. Digested lysates (400 μl each) were loaded on a sucrose

(9) TET2 and TET3 loss disrupts small intestine differentiation and homeostasisNature CommunicationsJuly 6, 2023Ihab Ansari, Llorenç Solé-Boldo, Meshi Ridnik, Julian Gutekunst, Oliver Gilliam, Maria Korshko, Timur Liwinski, Birgit Jickeli, Noa Weinberg-Corem, Michal Shoshkes-Carmel, et al.E. coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616, 2 mM Vanadyl-ribonucleoside complex, NEB S1402S) mixed with 1:3000 dilution of specific probe libraries and were transferred to an overnight incubation

(10) Single-cell quantification of ribosome occupancy in early mouse developmentNatureJune 29, 2023Hakan Ozadam, Tori Tonn, Crystal M. Han, Alia Segura, Ian Hoskins, Shilpa Rao, Vighnesh Ghatpande, Duc Tran, David Catoe, Marc Salit, et al.and incubated for 30 min at 37 °C. Digestions were stopped with 20 mM ribonucleoside vanadyl complex (S1402S, NEB). The samples were then loaded onto 20–50% sucrose gradients and ultracentrifuged in a SW41 Ti

(11) Multiplexed single-cell 3D spatial gene expression analysis in plant tissue using PHYTOMapNature PlantsJune 12, 2023Tatsuya Nobori, Marina Oliva, Ryan Lister, Joseph R. Ecker(Sigma-Aldrich, catalogue no. S6639); ribonucleoside vanadyl complex (New England Biolabs, catalogue no. S1402S); formamide (Sigma, catalogue no. F9037); RNase-free Tris buffer pH 8.0 (Invitrogen, catalogue no. AM9855G);

(12) DNA supercoiling restricts the transcriptional bursting of neighboring eukaryotic genesMolecular CellMay 18, 2023Heta P. Patel, Stefano Coppola, Wim Pomp, Umberto Aiello, Ineke Brouwer, Domenico Libri, Tineke L. LenstraArthrobacter luteus (powder),Sigma-Aldrich,Cat# L2524 Ribonucleoside Vanadyl Complex (RVC; liquid),NEB,Cat# S1402S Formamide (deionized),Sigma-Aldrich,Cat# F9037 "UltraPure™ SSC, 20X",Thermo Fisher Scientific,Cat#

(13) Single-cell detection of primary transcripts, their genomic loci and nuclear factors by 3D immuno-RNA/DNA FISH in T cellsFrontiers in ImmunologyMay 4, 2023Eralda Salataj, Charalampos G. Spilianakis, Julie Chaumeil40U/ml",InvitrogenTM (Thermo Fisher Scientific),AM2682 "RVC (Ribonucleoside Vanadyl Complex), 200mM",NEB,S1402S "Salmon Sperm, 10mg/ml",InvitrogenTM (Thermo Fisher Scientific),15632011 "SDS, powder",InvitrogenTM

(14) Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitisPLoS biologyMay 1, 2023Egozi A, Olaloye O, Werner L, Silva T, McCourt B, Pierce RW, An X, Wang F, Chen K, Pober JS, et al.mg/mL E. coli tRNA Sigma R1753, 2×SSC, 0.02% BSA Ambion AM2616, 2 mM Vanadyl-ribonucleoside complex NEB S1402S) mixed with 1:3,000 dilution of probes. Hybridization mix was incubated with tissues for overnight in

(15) Spatiotemporally resolved transcriptomics reveals the subcellular RNA kinetic landscapeNature MethodsMay 1, 2023Jingyi Ren, Haowen Zhou, Hu Zeng, Connie Kangni Wang, Jiahao Huang, Xiaojie Qiu, Xin Sui, Qiang Li, Xunwei Wu, Zuwan Lin, et al.(Sigma-Aldrich, catalog no. S6639); ribonucleoside vanadyl complex (New England Biolabs, catalog no. S1402S); yeast tRNA (Invitrogen, catalog no. AM7119); SUPERase·In (Invitrogen, catalog no. AM2696); 5-EU (Invitrogen,

(16) Correction of Clcn1 alternative splicing reverses muscle fiber type transition in mice with myotonic dystrophyNature CommunicationsApril 7, 2023Ningyan Hu, Eunjoo Kim, Layal Antoury, Thurman M. Wheelert-RNA (Invitrogen product number 15401-011), 2 mM ribonucleoside vanadyl complex (NEB product number S1402S), and 1 µg/ml 2′-O-methyl-modified RNA CAG repeat probe 5′ labeled with Alexa647. Post-hybridization,

(17) Integrated transcriptome and proteome analysis reveals posttranscriptional regulation of ribosomal genes in human brain organoidseLifeMarch 29, 2023Sidhaye J, Trepte P, Sepke N, Novatchkova M, Schutzbier M, Dürnberger G, Mechtler K, Knoblich JA.Escherichia coli tRNA [Merck, 10109541001], 2 mM vanadyl ribonucleoside complex [New England Biolabs, S1402S], 2× SCC, 10% formamide [Merck, F9037], 0.5% BSA) containing 250 nM of the FISH probe at 37°C overnight.

(18) Cell context-dependent CFI-1/ARID3 functions control neuronal terminal differentiationCell ReportsMarch 28, 2023Yinan Li, Jayson J. Smith, Filipe Marques, Anthony Osuma, Hsin-Chiao Huang, Paschalis KratsiosEscherichia coli tRNA [ROCHE 10109541001], 2 mM vanadyl ribonucleotide complex [New England Biolabs S1402s], 0.2 mg/mL RNase-free BSA [Ambion AM2618], 10% formamide) and added to the worms. The hybridization

(19) The minimal intrinsic stochasticity of constitutively expressed eukaryotic genes is sub-PoissonianbioRxivMarch 17, 2023Douglas E. Weidemann, Abhyudai Singh, Ramon Grima, Silke Haufspheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 μM beta-mercaptoethanol), and 1.2–5 μL 100T zymolyase (US Biological Z1005; 10 mg/mL) were added

(20) LSM1-mediated Major Satellite RNA decay is required for nonequilibrium histone H3.3 incorporation into parental pronucleiNature communicationsFebruary 21, 2023Zhu J, Chen K, Sun YH, Ye W, Liu J, Zhang D, Su N, Wu L, Kou X, Zhao Y, et al.(Invitrogen, 10777–019), 100 μg/mL cycloheximide (Sigma–Aldrich, C7698) and 2 mM vanadyl ribonucleoside (NEB, S1402S). Ten percent of the lysate was collected as input, and the rest was incubated with ANTI-FLAG® M2 Affinity

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